Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens

Abdulkadir Musliu; Sulaimon Adebisi; Samira Arzika

doi:doi:10.11648/j.abb.20210902.11

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Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens

Abdulkadir Musliu, Sulaimon Adebisi, Samira Arzika

Published in Advances in Bioscience and Bioengineering (Volume 9, Issue 2)

Received: 15 April 2021 Accepted: 7 May 2021 Published: 20 May 2021

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Abstract

The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.

Published in	Advances in Bioscience and Bioengineering (Volume 9, Issue 2)
DOI	10.11648/j.abb.20210902.11
Page(s)	20-24
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antibiotic, Lactic Acid Bacteria, Pathogens, Multidrug Resistance, Animals

References

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[13]	Pan, D. and Yu, G. (2014). The relative effect of milk base, starter, and process on yogurt texture: A review. Critical Reviews in FoodScience and Nutrition, 44, 113-137.
[14]	Singh, B. R., Jyoti, J., Chandra, M., Babu, N. and Sharma, G. (2009). “Drug Resistance Patterns of Salmonella Isolates of Equine Origin from India.” The Journal of Infection in Developing Countries 3 (02). 67.
[15]	Tiwari, J. G., Chaudhary, S. P., Tiwari, H. K., Dutta, T. K., Saikia, P. and Hazarika, P. (2011). “Microbial Evaluation of Market Milk and Milk-Products of Mizoram, India with Special Reference to Staphylococcus Aureus.” The Indian Journal of Animal Sciences 81 (4).
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APA Style

Abdulkadir Musliu, Sulaimon Adebisi, Samira Arzika. (2021). Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Advances in Bioscience and Bioengineering, 9(2), 20-24. https://doi.org/10.11648/j.abb.20210902.11

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ACS Style

Abdulkadir Musliu; Sulaimon Adebisi; Samira Arzika. Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Adv. BioSci. Bioeng. 2021, 9(2), 20-24. doi: 10.11648/j.abb.20210902.11

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AMA Style

Abdulkadir Musliu, Sulaimon Adebisi, Samira Arzika. Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Adv BioSci Bioeng. 2021;9(2):20-24. doi: 10.11648/j.abb.20210902.11

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@article{10.11648/j.abb.20210902.11,
  author = {Abdulkadir Musliu and Sulaimon Adebisi and Samira Arzika},
  title = {Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {9},
  number = {2},
  pages = {20-24},
  doi = {10.11648/j.abb.20210902.11},
  url = {https://doi.org/10.11648/j.abb.20210902.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20210902.11},
  abstract = {The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.},
 year = {2021}
}

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TY  - JOUR
T1  - Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens
AU  - Abdulkadir Musliu
AU  - Sulaimon Adebisi
AU  - Samira Arzika
Y1  - 2021/05/20
PY  - 2021
N1  - https://doi.org/10.11648/j.abb.20210902.11
DO  - 10.11648/j.abb.20210902.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 20
EP  - 24
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20210902.11
AB  - The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.
VL  - 9
IS  - 2
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Author Information

Abdulkadir Musliu

Department of Science Technology, Waziri Umaru Federal Polytechnic, BirninKebbi, Nigeria
Sulaimon Adebisi

Department of Science Technology, Waziri Umaru Federal Polytechnic, BirninKebbi, Nigeria
Samira Arzika

Department of Science Technology, Waziri Umaru Federal Polytechnic, BirninKebbi, Nigeria

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Abdulkadir Musliu, Sulaimon Adebisi, Samira Arzika. (2021). Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Advances in Bioscience and Bioengineering, 9(2), 20-24. https://doi.org/10.11648/j.abb.20210902.11

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ACS Style

Abdulkadir Musliu; Sulaimon Adebisi; Samira Arzika. Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Adv. BioSci. Bioeng. 2021, 9(2), 20-24. doi: 10.11648/j.abb.20210902.11

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AMA Style

Abdulkadir Musliu, Sulaimon Adebisi, Samira Arzika. Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Adv BioSci Bioeng. 2021;9(2):20-24. doi: 10.11648/j.abb.20210902.11

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@article{10.11648/j.abb.20210902.11,
  author = {Abdulkadir Musliu and Sulaimon Adebisi and Samira Arzika},
  title = {Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {9},
  number = {2},
  pages = {20-24},
  doi = {10.11648/j.abb.20210902.11},
  url = {https://doi.org/10.11648/j.abb.20210902.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20210902.11},
  abstract = {The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.},
 year = {2021}
}

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TY  - JOUR
T1  - Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens
AU  - Abdulkadir Musliu
AU  - Sulaimon Adebisi
AU  - Samira Arzika
Y1  - 2021/05/20
PY  - 2021
N1  - https://doi.org/10.11648/j.abb.20210902.11
DO  - 10.11648/j.abb.20210902.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 20
EP  - 24
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20210902.11
AB  - The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.
VL  - 9
IS  - 2
ER  -

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